Procedures:

1. Using the funnel, pour 1/4 Cup of mustard powder in a 1 gallon bottle.

2. Fill the bottle with water leaving some room at the top for shaking.

3. Replace the cap and shake thoroughly for 1 minute.

4. Determine the sampling location. It should be a relatively level spot where the soil does not appear to be heavily compacted.

5. Lay the 4 rulers down on the ground creating a square.

6. Record the temperatures of the soil and air using the thermometer and use SensorPush to record the humidity on the data sheet and in EpiCollect.

7. Describe the surroundings on the data sheet and in EpiCollect.

8. Re-Shake the bottles before pouring to eliminate any settling.

9. Set a timer for 10 minutes. Slowly pour the bottle of the mustard powder solution inside your designated site, making sure to pour evenly and let the ground absorb the solution. If the solution starts flowing outside of the sampling square, stop pouring, wait a few minutes to let the solution soak into the soil and then resume pouring.

10. On the side of the square where mustard powder solution has not been poured, use the shovel to collect soil and use the "Key to Soil Texture by Feel" to determine what type of soil it is and record on the data sheet and EpiCollect.

11. If the worms start to surface, carefully remove the worms and place them into the strainer. Do not record and collect any worms that emerge outside the sampling square but make sure to note them in the other invertebrate section of the data sheet and the EpiCollect.

12. Spray the worms with water to remove the mustard powder solution and then place the worms into the bowl.

13. Using the field guide, identify the worms and record the types and amounts of worms on the data sheet and the EpiCollect.

14. Make sure that you have recorded all of the information on EpiCollect and the data sheet.

15. If you find worms and you want to collect them, fill up the container used to collect worms with the 91% Isopropyl Alcohol and dump the worms collected into the container.

16. Make a label for the container with the field number, the date collected, the project name, and the recorders' initials.

17. If any other invertebrates are found, record them on the data sheet and the EpiCollect and try to collect them using the petri dishes if possible.

18. Repeat Steps 1-17 for each location.